Point mutations and genomic deletions in CCND1 create stable truncated cyclin D1 mRNAs that are associated with increased proliferation rate and shorter survival.
نویسندگان
چکیده
A gene expression signature of tumor proliferation rate in mantle cell lymphoma (MCL) is an overriding molecular predictor of the length of survival following diagnosis. Many strongly proliferative MCL tumors have exceptionally high cyclin D1 mRNA levels and preferentially express short cyclin D1 mRNA isoforms. We demonstrate here that these short mRNAs are cyclin D1a isoforms with truncated 3'UTRs, not alternatively spliced cyclin D1b mRNA isoforms. Among 15 MCL tumors with truncated cyclin D1 mRNAs, 7 had genomic deletions in the CCND1 3'UTR region. In 3 others, CCND1 contained point mutations that created premature polyadenylation signals, giving rise to 1.5-kb mRNAs lacking most of the 3'UTR. Both types of genomic alteration created transcripts lacking mRNA destabilization elements present in the wild-type cyclin D1a mRNA. Premature polyadenylation due to a 3'UTR mutation also was present in the Z-138 MCL cell line, which expressed both truncated and full-length cyclin D1a mRNAs. In these cells, the half-life of the short cyclin D1a mRNA was much longer than that of the full-length mRNA. We conclude that alterations of CCND1 3'UTR structure can significantly increase its oncogenic effect and worsen the clinical course of MCL patients.
منابع مشابه
Point mutations and genomic deletions in Cyclin D1 create stable truncated mRNAs that are associated with increased proliferation rate and shorter survival in mantle cell lymphoma
Word Count: 199 Main Text Word Count: 4996
متن کاملPlenary paper Point mutations and genomic deletions in CCND1 create stable truncated cyclin D1 mRNAs that are associated with increased proliferation rate and shorter survival
Adrian Wiestner,1,2 Mahsa Tehrani,2 Michael Chiorazzi,1 George Wright,1 Federica Gibellini,2 Kazutaka Nakayama,2 Hui Liu,2 Andreas Rosenwald,3 H. Konrad Muller-Hermelink,3 German Ott,3 Wing C. Chan,4 Timothy C. Greiner,4 Dennis D. Weisenburger,4 Julie Vose,4 James O. Armitage,4 Randy D. Gascoyne,5 Joseph M. Connors,5 Elias Campo,6 Emilio Montserrat,6 Francesc Bosch,6 Erlend B. Smeland,7 Stein K...
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ورودعنوان ژورنال:
- Blood
دوره 109 11 شماره
صفحات -
تاریخ انتشار 2007